Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences HEPES sodium salt 99.5 tit1KG
HEPES sodium salt 99.5 tit1KG
IBI SCIENTIFIC 3P (EDTA) SOLUTION, 100 mL
IBI 0.5MEDTA solution (pH 8.0)is ideal for biochemistry and molecular biology applications requiring a chelator of divalent metal ions. Ethylenediamine tetraacetic acid solution can be used to elucidate the role of antigen retrieval in immunostaining of ethanol fixed smears.
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles1L
DULBECCOS Dulbeccos Modified Eagles1L
Andwin Scientific VWR PBS-T BUFFER POWDER 100/CS
VWR® Buffer Powder Packs can save time and money, as there is no need to measure and weight many separate chemical components which is required when making buffers from scratch.Buffer powder quickly dissolves to make 1L of 1X solutionNo weighing or measuring of separate componentsNo pH adjustments requiredEconomical shipping, long shelf lifeQuality and consistency guaranteedVWR® Pre-measured buffer packs are extremely easy to use and are formulated so each pack makes 1 Liter of 1X buffer. Simply tear open a pack and mix completely with 1 liter of distilled water.Shipping costs are also minimal, as each case of 50 or 100 packs is compact and weighs only 2.5 Lbs.
eMolecules TES | 7365-44-8 | MFCD00007532 | 1g
Oakwood Chemical | TES | 1g | 537724648 | M05749 | | 7365-44-8 | MFCD00007532 | 229.250 | C6H15NO6S
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Abcam Antigen Retrieval Buffer (100 x Citrate Buffer), 250ML
Antigen retrieval 100X citrate buffer (pH 5.1 - 5.5) is a special solution that enables rehydration and antigen retrieval to be performed in formalin-fixed, paraffin-embedded tissue sections with one step. - Cited in over 25 publications - Contains citrate buffer, 1.5% 2-butoxyethanol, 1% hydrochloric acid - Optimized for citrate buffer-treated antibodies - Contains detergent for paraffin emulsification
The product is subject to the following: Abcam Restricted Use Statement
![Research Products International Corp TRIS Hydrochloride [Tris(hydroxymethyl) aminomethane HCl], 5 Kilograms](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-T60050-5000.0.JPG-250.jpg)
Research Products International Corp TRIS Hydrochloride [Tris(hydroxymethyl) aminomethane HCl], 5 Kilograms
TRIS Hydrochloride, Tris-HCl, is a buffering agent commonly utilized to maintain a stable pH in experimental procedures.
It is primarily used as a buffering agent for applications that require a slightly alkaline to neutral pH range. Also, It is effective in the pH range of 7.2 to 9.0, making it suitable for a variety of biochemical and molecular biology techniques.
TRIS HCl is commonly used in DNA and RNA extraction, protein purification, enzyme assays, and electrophoresis. It helps maintain the desired pH conditions for enzymatic reactions, stability of nucleic acids, and separation of proteins during electrophoresis.
One of the advantages of TRIS HCl is its ability to resist changes in pH caused by the addition of acids or bases, making it a reliable buffering agent. It also exhibits low ionic strength, which minimizes interference with biological systems and enzymatic activities.
Medchemexpress LLC Tapso | 68399-81-5 | 98.0% | 100 G
TAPSO is a widely used biological buffer and pH stabilization reagent. It offers an effective pH range between 7 and 8, with pKa values typically falling between 7.5 and 9.0. This compound contains Tris groups and has been observed to exhibit reactive activity with zwitterionic glycine peptides. It is primarily intended for research applications.
- Effective pH range of 7-8.
- pKa values ranging from 7.5-9.0.
- Contains Tris groups.
- Shows reactive activity with zwitterionic glycine peptides.
- Suitable for various research applications.
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles1L
This modification has been shown to improve the stability of the medium.
Abcam PBS Tablets (1000 ml), 100TABLET
MW 411.04 Da. One of the most commonly used buffers provided in a convenient, tablet form. PBS is isotonic and non-toxic to cells and has the ability to maintain their osmolarity thereby making the buffer suitable for washing procedures in cell cultures and for immunoassays.
The product is subject to the following: Abcam Restricted Use Statement
